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发布于:2018-3-9 07:41:00  访问:23 次 回复:0 篇
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Edwurst sausage and liver p Isolates from samples from the initial
Edwurst sausage and liver p Isolates from samples on the RG7388 biological activity initial products identified to be optimistic for L. Incidence per age group of listeriosis together with the outbreak PFGE profile, SwedenAge group (years) Total Population , ,, Situations Incidence per , V. DAHL ET AL.corporation resumed production for commercial purposes after thorough cleaning in the production line and sampling of products and also the environment prior to release, but then stopped once more on February when L. monocytogenes was located in sliced liver p sliced smoked ham and sliced cooked medwurst sausage. At this time, the National Meals Agency received isolates from environmental and food samples from the producer. Of samples analysed, 5 were identified to have a equivalent profile to the outbreak PFGE profile. The producer then closed the production line and dismantled it and slicing of coldcuts was transferred to an additional production plant belonging for the similar firm. Fiftyone patient isolates from the outbreak type were wholegenome sequenced and all isolates were found to possess identical PFGE profiles. Additionally, historical isolates from and together with the outbreak PFGE profile had been sequenced. In addition to the 5 isolates from coldcuts, seven isolates with the outbreak PFGE profile from other European countries in were also included. These originated from meals (shrimp, brie, hamburgers and pork) and from swab samples from a slicing machine. One isolate was supplied by the Federal Institute for Danger Assessment in Germany (BfR, Sylvia Kleta), and six by the Austrian Agency for Well being and Food Safety (AGES, Ariane Pietzka). Wholegenome sequence data (raw reads) from one isolate from a Danish patient using the outbreak PFGE profile, diagnosed just before the Swedish outbreak, was added in the cluster analysis so that you can ascertain whether or not that case was also a part of the outbreak (offered by Jonas Larsson, Statens Serum Institut, Denmark). From the Swedish situations, isolates from constituted the main cluster (separated by significantly less than single nucleotide polymorphisms (SNPs)) inside the phylogenetic tree (Figure) and of them had been identical. Ten differed by one to two SNPs and three differed by more than SNPs from the principal outbreak cluster. The 5 isolates from the coldcut producer have been identical and clustered with one particular patient outbreak isolate. This cluster was separated by eight SNPs in the most important cluster. Of the Swedish historical patient isolates from the outbreak PFGE variety, seven have been identical for the main outbreak cluster and three differed by a single to 3 SNPs (Figure). The seven nondomestic food and slicing machine isolates differed by far more than SNPs in the most important cluster. Among the outliers from the outbreak patient isolates clustered using the Danish patient isolate. Figure . Minimum spanning tree of outbreak patient and food isolates (defined by PFGE as within the case definition). Each and every circle represents 1 isolate and each branch represents 1 singlenucleotide polymorphism (SNP) unless otherwise stated using a number within a circle or next to a branch. Patient outbreak isolates are shown in yellow and domestic food isolates from coldcuts with all the outbreak PFGE profile in purple.Fiftyone instances in Sweden have been identified as belonging to this outbreak (or on excluding the three isolates that have been not a part of the outbreakaccording to WGS), creating it certainly one of the biggest L.Edwurst sausage and liver p Isolates from samples of the initial merchandise located to become constructive for L.
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