网站标志
会员登录信息
您好,欢迎光临!   [请登录]   [免费注册]
商品搜索
点评详情
发布于:2018-6-6 06:33:51  访问:42 次 回复:0 篇
版主管理 | 推荐 | 删除 | 删除并扣分
N, the sum of OD captured the differences in staining intensity
A majority of the patient tumor samples (65/87, 75 ) used in the present study showed < 60.0 pg bFGF/mg protein.Comparison of visual scoring and imaged analysis results Table 1 compares the results obtained using the computerized image analysis results to our earlier results established PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27104741 using the conventional, visual scoring APD334 biological activity method. bFGF levels in tumors were significantly and inversely correlated with tumor sensitivity toANegative Control PC3 HT29 FADU MiaPaCa -BELISA Result (pg bFGF/mg protein)200 150 100 50 0 0.0 0.1 0.2 0.Sum of OD/AreaFigure 2 bFGF standard curve bFGF standard curve. (A) Staining of bFGF (brown color) in PC3, HT29, FaDu, and MiaPaCa-2 tumors. Counter-stained with hematoxylin (blue). 400?magnification. The corresponding respective bFGF levels measured using ELISA were 39.0, 57.3, 60.0, and 208 pg/mg total protein. Negative control: MiaPaCa-2 tumor stained with nonspecific, IgG antibody. (B) bFGF standard curve (y = 690.5x; r2 = 0.996, p < 0.001). Mean ?Standard deviations.Page 5 of(page number not for citation purposes)Journal of Translational Medicine 2008, 6:http://www.translational-medicine.com/content/6/1/Table 1: Relationship between bFGF level and paclitaxel activity: Comparison of image analysis and visual scoring results.N, the sum of OD captured the differences in staining intensity and the sum of the positively-stained areas. For example, HT29 and FaDu tumors showed veryHead and NeckBreastProstateBladderOvarianFigure 1 bFGF staining in patient tumors bFGF staining in patient tumors. Surgical tumor specimens from patients (n = 87, untreated) were stained for bFGF as described in Materials and Methods. Representative samples of each tumor type are shown. 400?magnification.Page 4 of(page number not for citation purposes)Journal of Translational Medicine 2008, 6:http://www.translational-medicine.com/content/6/1/different bFGF staining patterns, i.e., punctuated, high intensity staining in HT29 vs diffused and less intense staining in FaDu (Figure 2A). But these tumors showed similar sums of OD, in agreement with the similar total bFGF levels measured by ELISA. Furthermore, the sums of OD were linearly correlated with the bFGF levels measured by ELISA, in 4 tumor types (Figure 2B; y = 690.5x; r2 = 0.996, p < 0.001). Subsequent analysis used the sum of OD parameter. Figure 2B shows a representative standard curve, covering the range of 0 to 208 pg bFGF/mg protein. Three of the four tumors used to obtain the standard curve showed a narrower bFGF concentration range of 0?0 pg/mg protein. The highest level of 208 pg/mg was derived from MiaPaCa-2 tumor. Exclusion of the MiaPaCa-2 tumor did not significantly alter the r2 value. A majority of the patient tumor samples (65/87, 75 ) used in the present study showed < 60.0 pg bFGF/mg protein.Comparison of visual scoring and imaged analysis results Table 1 compares the results obtained using the computerized image analysis results to our earlier results established PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27104741 using the conventional, visual scoring method. For bFGF levels in individual tumors, the results PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/19370553 from the two methods show a significant, positive correlation (r2 = 0.354, p < 0.0001). For the relationship between bFGF levels and paclitaxel sensitivity in individual tumors, both methods yielded significant correlation, but the image analysis results showed 2? times higher r2 values. Relationship between bFGF level and paclitaxel sensitivity in individual patient tumors Data on tumor sensitivity to the antiproliferation and apoptotic effects of paclitaxel were obtained from our previous report [20]. The antiproliferative effects were quantified in terms of the drug concentration required to produce 30 inhibition of bromodeoxyuridine labeling index (IC30) and the maximum inhibition of the same parameter (EMAX).
共0篇回复 每页10篇 页次:1/1
共0篇回复 每页10篇 页次:1/1
我要回复
回复内容
验 证 码
看不清?更换一张
匿名发表 
当前位置
脚注信息
Copyright ? 2009-2012 All Rights Reserved. 苏州市早晨空气电器有限公司 版权所有   沪ICP备01234567号
服务时间:周一至周日 08:30 — 20:00  全国订购及服务热线:0512-85169714 
联系地址:江苏省吴江市松陵镇伟业大厦3楼12号   邮政编码:215200