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发布于:2018-6-12 11:13:43  访问:53 次 回复:0 篇
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Chanically ventilated barns from 4:00 to eight:00 am, as well as from 16:00 to 20:00 pm.
Two mechanically ventilated goose barns with an automatic lighting system were applied to host the ganders used within the study. On March 1, 2015, a flock of Yangzhou goose ganders (n = 210) of 112 days of age plus the identical genetic origin have been equally divided into two groups: A and B. Group A ganders had been initially exposed to a quick photoperiod of eight h (8L:16D) for 56 days, from March 1, 2015, to April 25, 2015 (Fig. 2a). Subsequently, the photoperiod was increased to 12 h (12L:12D) for 218 days, from April 26, 2015, to November 29, 2015 (Fig. 2a). Inside the final phase, the each day photoperiod was further enhanced to 16 h (16L:8D) for 38 days, from November 30, 2015, until the finish with the experiments on January six, 2016 (Fig. 2a). Group B ganders alsoOn days 51, 67, 88, 131, 205, 250, and 313 of your experiment, eight ganders from each group had been randomly chosen and slaughtered for tissue sample collection. Immediately after collection, PP58 biological activity tissues in the hypothalamus, pituitary, and testes have been snap frozen in liquid nitrogen, and stored at -80 until gene NVP-BEZ235 site expression assays had been performed. A piece of testicular tissue ( 0.125 cm3) was sliced from the left testis of every single gander and was promptly fixed in ten buffered neutral formalin resolution for 24 h, and subsequently applied for histology research employing an automated tissue processor (Shandon Excelsior ES, P09046, ThermoScientific, Germany). The processing involved common step-wise dehydration with alcohol of increasing concentrations (50, 60, 95 , and absolute alcohol, respectively), clearing in xylene (two adjustments), infiltration, and embedding in molten paraffin wax.Zhu et al. Frontiers in Zoology (2017) 14:Web page four ofFig. two Plasma concentrations of testosterone (c and d), triiodothyronine (e PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27484364 and f), and thyroxine (g and h) in ganders beneath two artificial photoperiods. Vertical bars indicate common error of the imply. The artificial photoperiod treatment (a) consisted of a short photoperiod of eight h (8L:16D) for 56 days from March 1, 2015 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/29069523 till April 25, 2015, followed by a long photoperiod of 12 h (12L:12D) for 218 days from April 26, 2015 till November 29, 2015, and completed using a long photoperiod of 16 h (16L:8D) from November 30, 2015 till the finish on the experiment, on January six, 2016.Chanically ventilated barns from four:00 to 8:00 am, and also from 16:00 to 20:00 pm. For provision of your 12-hour photoperiod, the ganders had been confined inside the barns from four:00 to 6:00 am, and also from 18:00 to 20:00 pm. The 16-hour extended photoperiod therapy consisted of the organic illumination throughout the daytime plus the supplementary illumination of 80 to 100 lux by fluorescent tubes at times after sunset and ahead of sunrise. The birds had been fed ad libitum with mixed feed of 12.5 crude protein, supplemented with green grass anytime doable. Feed was given for the duration of daytime, but ganders always had absolutely free access to drinking water. Through the course in the experiment, blood samples were collected via wing veins into heparinized syringes just about every 14 days.
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