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发布于:2018-11-6 11:09:18  访问:30 次 回复:0 篇
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Ed in patients with severe sepsis and septic shock. The aim
The aim of the present investigation was to evaluate the induction of tolerance in monocytes of healthy volunteers, in entire blood, immediately after LPS exposition in vitro, assessed by intracellular cytokine detection and reactive oxygen species (ROS) generation. Methods Peripheral blood cells had been conditioned with compact doses of LPS for 18 hours and challenged PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27864321 with diverse agonists of Tolllike receptors (macrophage-activating lipopeptide-2, flagellin and LPS) and whole Gram-negative (Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) killed bacteria. For detection of intracellular IL-6, samples of entire blood have been stimulated for 6 hours. Monocytes had been identified by forwardscatter and side-scatter parameters and CD14-positive staining. The samples had been stained to confirm the intracellular production of IL-6 on monocytes by flow cytometry. For induction of ROS, entire blood was stimulated for 30 minutes with LPS, P. aeruginosa and S. aureus. ROS production was measured by flow cytometry, applying two,7-dichlorfluorescein-diacetate detection. Benefits The conditioning with rising doses of LPS resulted in decrease intracellular detection of IL-6 in monocytes PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28300835 after the challenge with LPS. A comparable impact was observed with macrophage-activating lipopeptide-2, P. aeruginosa, and S. aureus, but not with flagellin. LPS conditioning with 15 ng/ml LPS, alternatively, resulted in preserved or enhanced production of ROS in monocytes after challenge with LPS, P. aeruginosa and S. aureus. Conclusion The phenomenon of tolerance entails a complex regulation, in which the production of proinflammatory cytokines, which include IL-6, is diminished, whereas the production of ROS is preserved and even improved.P47 Validation of polymerase chain reaction in experimental sepsis diagnosis beyond blood culture Marcello Ruiz-Silva, Derci Sa-Filho, Marcos Caseiro, Ivan Koh Departments of Pediatric and Molecular Biology of Lus da University Center and Division of Surgery of Federal University of Sao Paulo, Sao Paulo, Brazil Critical Care 2008, 12(Suppl 5):P47 (doi: 10.1186/cc7080) Background A optimistic blood culture (BC) is regarded the goldstandard strategy for sepsis diagnosis, though its sensibility is low (ten to 30 ) ?which demands a far better diagnostic tool to limit broad-spectrum antibiotic use within the majority of individuals without the need of culture-based sepsis diagnosis.Ed in individuals with severe sepsis and septic shock. The aim of the present investigation was to evaluate the induction of tolerance in monocytes of wholesome volunteers, in entire blood, following LPS exposition in vitro, assessed by intracellular cytokine detection and reactive oxygen species (ROS) generation. Conclusion The phenomenon of tolerance CS-0196 site involves a complex regulation, in which the production of proinflammatory cytokines, for instance IL-6, is diminished, whereas the production of ROS is preserved and even enhanced.P47 Validation of polymerase chain reaction in experimental sepsis diagnosis beyond blood culture Marcello Ruiz-Silva, Derci Sa-Filho, Marcos Caseiro, Ivan Koh Departments of Pediatric and Molecular Biology of Lus da University Center and Department of Surgery of Federal University of Sao Paulo, Sao Paulo, Brazil Critical Care 2008, 12(Suppl five):P47 (doi: ten.1186/cc7080) Background A good blood culture (BC) is viewed as the goldstandard method for sepsis diagnosis, while its sensibility is low (ten to 30 ) ?which demands a far better diagnostic tool to limit broad-spectrum antibiotic use within the majority of individuals with no culture-based sepsis diagnosis.
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